United Kingdom (UK)
Recombinant monoclonal antibody to GABA(B)R2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N81/2.
UniProt Accession Number of Target Protein: O75899
Alternative Name(s) of Target: GABA-B receptor 2; GABA-B-R2; GABA-BR2; GABR2; Gb2; GABBR2; GPR51; GPRC3B; HG20; HRIHFB2099; Gamma-aminobutyric acid type B receptor subunit 2; G-protein coupled receptor 51; EIEE59; NDPLHS; DEE59; N81/2.2
Immunogen: The original antibody was generated by immunizing Balb/c mice with fusion protein amino acids 862-913 (cytoplasmic C-terminus) of human GABABR2.
Specificity: This antibody is specific for human GABABR2 and cross-reacts with mouse and rat GABABR2. It does not cross-react with GABABR1.
Application Notes: This antibody can be used for IB, ICC, and IHC (RRID: AB_2877276). Independently, it was used for WB (Pacey et al., 2011; PMID: 21215802, 21636656) (Kang et al., 2017; PMID: 28213518) and bioorthogonal non-canonical amino acid tagging - proximity ligation assay (BONCAT-PLA) (Workman et al., 2015; PMID: 25560757).
Antibody first published in:
Pacey et al. Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse. Mol Cell Neurosci. 2011 Mar;46(3):563-72. doi: 10.1016/j.mcn.2010.12.005. PMID:21215802
Note on publication:
The original publication investigates the role of the regulator of G-protein signaling 4 (RGS4) in fragile X syndrome (FXS) by generating FXS/RGS4 double knockout mice.