United Kingdom (UK)
Recombinant monoclonal antibody to Gelsolin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes GsnVHH 11 (Nb11).
UniProt Accession Number of Target Protein: P06396
Alternative Name(s) of Target: GSN; AGEL; ADF; Actin-depolymerizing factor; Brevin
Immunogen: The original antibody was generated by immunizing a llama subcutaneously with human recombinant gelsolin.
Specificity: This antibody recognizes gelsolin irrespective of calcium and completely blocks its interaction with G-actin. This antibody specifically recognizies and epitope in the G2-G3 domain of gelsolin.
Application Notes: Gelsolin is a multifunctional actin-binding protein that controls cellular actin organization and migration. The original VHH version of this antibody binds gelsolin with a Kd of ~5 nM irrespective of calcium and completely blocks its interaction with G-actin. The specificity of the antibody towards gelsolin was confirmed with ELISA. GsnVHH 13 expressed as intrabodies immunoprecipitate endogenous gelsolin. His-tagged GsnVHH 13 can be used to identify gelsolin in a western blot assay. This antibody can trace gelsolin in membrane ruffles of EGF-stimulated MCF-7 cells and delay cellmigration without affecting F-actin severing/capping oractin nucleation activities by gelsolin as demonstrated by immunofluorescense staining. It was also reported that free gelsolin is targeted to the nucleus by nuclear localizationsequence (NLS)-GsnVHH 11 in HeLa cells (PMID:20140750). Hereditary gelsolin amyloidosis is an autosomal dominantly inherited amyloid disorder caused by a point mutation in the GSN gene resulting in disturbed calcium binding and improper folding of the second gelsolin domain (G2) and rendering the mutant plasma gelsolin susceptible to a proteolytic cascade. This antibody was also used as a molecular chaperone to protect mutant plasma gelsolin from furin proteolysis in the trans-Golgi network (PMID: 25601851). This antibody was also used in the study of structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant (PMID: 30625383).
Antibody first published in:
Abbeele et al. A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction. Cell Mol Life Sci. 2010 May;67(9):1519-35. PMID:20140750
Note on publication:
Describes the generation, characterization, epitope mapping and crystal structure of this antibody.