United Kingdom (UK)
Recombinant monoclonal antibody to Genome polyprotein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma C5.
UniProt Accession Number of Target Protein: P03303
Alternative Name(s) of Target: P1; Capsid protein VP3; Virion protein 3
Immunogen: The original antibody was raised by immunizing a mouse with human rhinovirus 14.
Specificity: This antibody is specific for capsid protein VP3, NIm-III site of Human rhinovirus 14.
Application Notes: The structure of the original antibody bound to its antigen was determined using cryo-EM. Further the original antibody was used for both a preattachment neutralizing assay and a postattachment neutralization assay on HeLa-H1 cell monolayers. The antibody showed the ability to neutralize RV-B14 in both assays. The antibody was able to trigger genome release in the virus (Dong et al., 2017; PMID:28696310).
Antibody first published in:
Dong et al. Antibody-induced uncoating of human rhinovirus B14 Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):8017-8022. PMID:28696310
Note on publication:
The original paper reports that the antigen-binding (Fab) fragment of a neutralizing antibody (C5) can trigger genome release from RV-B14 to form emptied particles and neutralize virus infection.