United Kingdom (UK)
Recombinant monoclonal antibody to Genome polyprotein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma E104.
UniProt Accession Number of Target Protein: P29990
Alternative Name(s) of Target: DV2-104; Capsid protein C; Core protein; Protein prM; Peptide pr; Small envelope protein M; Matrix protein; Envelope protein E; Dengue virus type 2
Immunogen: The original antibody was raised by immunizing a C57BL/6 mouse with DENV-2.
Specificity: This antibody is specific for DIII C-C′ loop of Envelope protein E of Dengue virus type 2
Application Notes: The original antibody was used for a plaque reduction assay on DENV-2 (Zhang et al., 2015; PMID:25355881, Sukupolvi-Petty et al., 2010; PMID:20592088). The Fab was incubated with DENV-2 and then added to BHK21 cells. The structure of the original antibody was determined using cryo-EM (Zhang et al., 2015; PMID:25355881). The original version of this antibody was used for flow cytometry on DENV infected cells. This antibody was used for an in vivo protection assay on BALB/c mice. Here the mice were given the antibody after infection with the DENV-2. It showed improvement over control (Sukupolvi-Petty et al., 2010; PMID:20592088). This antibody was used for an ELISA on immature virus particles. This antibody was able to promote infectivity of DENV-2. In short it made normally uninfectious immature virus infectious (da Silva Voorham et al., 2012; PMID:22431958).
Antibody first published in:
Sukupolvi-Petty et al. Structure and function analysis of therapeutic monoclonal antibodies against dengue virus type 2 J Virol. 2010 Sep;84(18):9227-39. PMID:20592088
Note on publication:
The original paper investigated in detail the functional activity of a large panel of new anti-DENV-2 mouse MAbs