Anti-GFP [GBP1 (Enh)]

Description:

Recombinant monoclonal antibody to GFP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the alpaca peripheral lymphocyte GBP1 (Enh).

Clone Name:

GBP1 (Enh)

Published Application(s):

in vitro, in vivo, IF

Published Species Reactivity:

Species independent

UniProt Accession Number of Target Protein: P42212

Alternative Name(s) of Target: Green fluorescent protein; GFP Binding Protein 1; Enhancer
Immunogen: The original antibody was generated by immunizing an alpaca with GFP.

Specificity: This antibody is specific for GFP.

Application Notes: The Enhancer (Enh) anti-GFP nanobody, also known as GFP binding protein 1 (GBP1), was specifically used for manipulating protein conformation in vitro and in vivo; this nanobody caused a fourfold fluorescence enhancement by stabilizing the deprotonated, negatively charged state of the GFP chromophore, resulting in increased fluorescence intensity (Kirchhofer et al., 2010; PMID: 20010839).

Antibody first published in: Kirchhofer et al. Modulation of protein properties in living cells using nanobodies Nat Struct Mol Biol. 2010 Jan;17(1):133-8. doi: 10.1038/nsmb.1727. Epub 2009 Dec 13 PMID:20010839
Note on publication: The original publication explores the use of camelid-derived nanobodies to modulate the conformation and spectral properties of green fluorescent protein (GFP) in living cells, revealing the potential for manipulating and studying protein conformations using nanobodies.

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