United Kingdom (UK)
Recombinant monoclonal antibody to GFP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Minimizer.
UniProt Accession Number of Target Protein: P42212
Alternative Name(s) of Target: Green fluorescent protein; MI; GBP4
Immunogen: The original antibody was generated by immunizing camels with GFP. A phage display library was constructed and the antibody was selected by panning against GFP.
Specificity: The antibody is specific for green fluorescent protein. The antibody interacts with β-strands 6 and 7 of GFP.
Application Notes: The antibody could reversibly reduce GFP fluorescence by a factor of 5 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP–antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.45 nM) (Kirchhofer et al., 2010; PMID: 20010839). Fluorescence modulation in living cells was also observed after the addition of the antibody to HeLa cells expressing GFP (Schmidthals et al., 2010; PMID: 20372881).
Antibody first published in:
Kirchhofer et al. Modulation of protein properties in living cells using nanobodies. (2010) Nat Struct Mol Biol 17: 133-138 PMID:20010839
Note on publication:
The original paper describes the generation and characterization of the antibody