United Kingdom (UK)
Recombinant monoclonal antibody to Gliadin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes VHH26.
UniProt Accession Number of Target Protein: Q306G0
Alternative Name(s) of Target: Alpha-gliadin; α-gliadin; Celiac Allergen; Coeliac Allergen
Immunogen: The original antibody was prepared by immunization of Llama glama with whole gliadin.
Specificity: This antibody specifically recognizes and binds and epitope unique to wheat alpha gliadins.
Application Notes: This antibody specifically binds an epitope unique for wheat alpha gliadins which is not present in gamma or omega gliadins. It does not cross reat with rye or barley gliadins. VHH26 can detect gliadins in denaturing conditions and it retains its binding capacity in presence of 15% ethanol, 0.5% 2-mercaptoethanol, and 0.5 M guanidinium chloride. Capture ELISA using VHH26 was able to detect gliadins in samples shown as negatives by conventional ELISA. This antibody can be used for detection of gluten in commercial food samples using ELISA (limit of detection 0.24 ppm) and by competitive ELISA using conventional antibodies (limit of detection: 1 ppm) (PMID: 20039674).
Antibody first published in:
Doña et al. Single domain antibodies are specially suited for quantitative determination of gliadins under denaturing conditions. J Agric Food Chem (2010); 58(2): 918-26. PMID:20039674
Note on publication:
Describes the generation and specific use of this antibody in the quantitive determination of gliadins.