United Kingdom (UK)
Recombinant monoclonal antibody to glycoprotein E. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 19/1786.
UniProt Accession Number of Target Protein: Q01299
Alternative Name(s) of Target: tick-borne encephalitis virus; TBEV; envelope domain III; EDIII; envelope protein
Immunogen: The original antibody was generated by immunizing mice against the tick-borne encephalitis (TBE) virus, strain K23.
Specificity: This antibody is reported to bind amino acids 306-339 of the immunodominant loop structure of the glycoprotein E of tick-borne encephalitis virus. The antibody does not cross react with other flaviviruses. Tick-borne encephalitis virus (TBEV) is a member of the tick-borne flaviviruses (TBFVs) in the family Flaviviridae which cause encephalomeningitis and encephalitis in humans and other animals.
Application Notes: The specificity of the antibody for glycoprotein E was confirmed by ELISA analysis. The antibody detected glycoprotein E by western blot analysis (Niedrig et al., 1994; PMID: 7817895). Neutralization of TBVE by the original format of the antibody and Fab fragment was determined, the EC50 values were respectively 0.24 ± 0.03 and 35.0 ± 2.5 µg ml−1. The cryo-EM structure of the Fab fragment in complex with Tick-borne encephalitis virus was determined. A fusion-from-without assay using C6/36 cells was performed to determine if the original format of the antibody and the Fab can prevent membrane fusion in vivo. Results showed that the native TBEV induced cell fusion at low pH, the virus in complex with the original format of the antibody lost this ability, and the virus in complex with the Fab induced cell fusion with lower efficiency than the native virus. The antibody has therapeutic potential (Fuzik et al. 2018; PMID: 29382836).
Antibody first published in:
Niedrig et al. Monoclonal antibodies directed against tick-borne encephalitis virus with neutralizing activity in vivo. Acta Virol. 1994 Jun;38(3):141-9. PMID:7817895
Note on publication:
The paper describes the generation and characterization of a panel of antibody specific for tick-borne encephalitis virus.