United Kingdom (UK)
Recombinant monoclonal antibody to GPIIB/IIIA. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody PDG7.
Alternative Name(s) of Target: glycoprotein IIb/IIIa; integrin αIIbβ3;
Immunogen: A phagemid library was constructed from the peripheral blood lymphocytes of a donor who was immunised with RhD+ red blood cells, and PDG7 was isolated after five rounds of affinity selection.
Specificity: PDG7 does not bind to antigen denatured by heat of pH, indicating the necessity of intact GPIIb/IIIa.
Application Notes: Dot blots confirmed the specificity of PDG7 for GPIIB/IIIA. Inhibition assays were used to show that platelet-associated autoantibodies from AITP patients binds a closely related epitope to that of the recombinant anti-GPIIb/IIIa phage clones (Escher et al, 1998). Inhibition studies also confirmed that PDG7 inhibited binding of eluted platelet-associated autoantibodies from AITP patients to platelets (Escher et al, 1998).
Antibody first published in:
Escher et al, 1998. Recombinant human natural autoantibodies against GPIIb/IIIa inhibit binding of autoantibodies from patients with AITP Br J Haematol. 1998 Aug;102(3):820-8. PMID:9722313
Note on publication:
Describes the production of the phage library and the characterisation of PDG7 through dot blots and inhibition assays.