United Kingdom (UK)
Recombinant monoclonal antibody to HBV pre-S1 protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MA18/7.
UniProt Accession Number of Target Protein: Q67886
Alternative Name(s) of Target: PreS1; Large envelope protein; L glycoprotein; L-HBsAg; Large S protein; Large surface protein; Major surface antigen
Immunogen: The original antibody was generated by immunizing mice with HBsAg subtype ayw.
Specificity: This antibody binds a clearly defined epitope 'DPAF' (amino acids 31-34) of the pre-S1 region of the hepatitis B virus. Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus (HBV). It can cause chronic infection and puts people at high risk of death from cirrhosis and liver cancer.
Application Notes: This antibody can be used for the identification of HBV pre-S2 protein using immunoblot and western blot (PMID: 6492255). The original mouse IgG1 version of this antibody was reported to bind pre-S1 protein of HBV with a binding affinity of Kd= 0.24 nM (PMID: 10509818). Immunoblot analysis revealed that the tetrapeptide Asp-Pro-Ala-Phe (DPAF), located at the position preS(31–34) and conserved in all known HBV genomes, is sufficient to bind MA18/7 antibody (PMID: 1279369). It was reported that MA18/7 inhibits the binding of natural HBV to hepatocyte membranes (PMID: 7964611). This antibody has shown to neutralize infectivity of HBV for primary human hepatocytes, also blocked infection of primary Tupaia belangeri hepatocytes (PMID: 12915565).
Antibody first published in:
Heermann et al. Large surface proteins of hepatitis B virus containing the pre-s sequence. J Virol. 1984 Nov;52(2):396-402. PMID:6492255
Note on publication:
Describes the identification and characterization of large surface protein of HBV using antibodies.