Anti-HDGF [H3]

Description:

Recombinant monoclonal antibody to HDGF. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma H3.

Clone Name:

Published Application(s):

IP, neutralizing, WB, IF, IHC

Published Species Reactivity:

Human, Mouse

Products based on this clone:

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Ab02457-23.0 Anti-HDGF [H3]
Human, Mouse
Rabbit IgG
Purified
In Stock

Ab02457-10.0 Anti-HDGF [H3]
Human, Mouse
Human IgG1
Purified
Ships in 4-5 weeks

Ab02457-1.1 Anti-HDGF [H3]
Human, Mouse
Mouse IgG1
Purified
In Stock

Ab02457-10.3 Anti-HDGF [H3]
Human, Mouse
Human IgG1
Fc Silent™
Purified
In Stock

Ab02457-2.0 Anti-HDGF [H3]
Human, Mouse
Mouse IgG2a
Purified
In Stock

Ab02457-2.3 Anti-HDGF [H3]
Human, Mouse
Mouse IgG2a
Fc Silent™
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P51858

Alternative Name(s) of Target: Hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2
Immunogen: The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody C1.

Specificity: This antibody is specific for the HDGF protein.

Application Notes: This antibody was used for immunoprecipitation on total cell lysates from H460 NSCLC cells (US20070243191). The neutralizing ability of this antibody was tested on A549. The result was a significant decrease in tumor size (US20070243191). This antibody was fluorescently labelled and injected into nude mice to test the antibody distribution. The antibodies evenly distributed in the animals including the tumors. This antibody entered into the cytoplasmic compartment of some tumor cells (US20070243191). Immunohistochemistry with this antibody was performed on tissues from NSCLC patients (US20070243191). Western blot was performed on HDGF protein using this antibody (Ren et al, 2009;pmid:19435872). ELISA was performed on the HDGF protein using this antibody (US20070243191).

Antibody first published in: Ren et al. Antibodies targeting hepatoma-derived growth factor as a novel strategy in treating lung cancer. Mol Cancer Ther. (2009); 8(5): 1106–1112. PMID:19435872
Note on publication: Describes the generation of anti-HDGF monoclonal antibodies and reports their anti-tumor activity in lung cancer xenograft models.

Western blot using Ab02457-1.1 Click on image for more details.

Western Blot using Anti-HDGF antibody H3 (Ab02457). HeLa(A) (0.0001ug/ml), HeLa nuclear (B) (0.0001ug/ml), MCF7(C) (0.0001ug/ml), MCF7 nuclear (D) (0.0001ug/ml), HepG2(E) (0.0001ug/ml), HepG2 nuclear(F) (0.0001ug/ml), A549(G) (0.0001ug/ml) and A431(H) (0.0001ug/ml) cell lysate (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric mouse version of H3 (Ab02457-1.1) 0.001µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

IF staining of MCF7 cells using Ab02457-1.1. Click on image for more details.

Immunofluorescence staining of MCF7 cells with Anti-HDGF (Ab02457) H3 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton stained with the chimeric mouse IgG1 version of H3 (Ab02457-1.1) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02457-1.1, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-1.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry analysis of MCF7 cells using Ab02457-1.1. Click on image for more details.

Flow cytometry using the Anti-HDGF antibody H3 (Ab02457). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of H3 (Ab02457-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.