United Kingdom (UK)
Recombinant monoclonal antibody to Hemoglobin monomer 14kDa. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 13F7.
Alternative Name(s) of Target: haemoglobin monomer; Hb; Hbg; PHb; mAb13F7
Immunogen: The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a mammalian blood immunogen.
Specificity: 13F7 recognizes a hemoglobin monomer (14 Da) in blood samples of various mammals (pig, sheep, horse, cow).
Application Notes: This antibody is recommended for the identification of hemoglobin and, more generally, blood in various samples and products, such as commercial meat products. It was used in the development of a successful ELISA-based assay for the quantification of hemoglobin (PHb) in meat products. It had a very wide working range from 0.5 ppm to 1000 pp, high sensitivity and precision (Jiang et al., 2018; pmid: 29412908). Testing by Western blot demonstrated that 13F7 is able to detect a hemoglobin monomer (mass: 14 Da) in blood samples of different mammals (Jiang et al., 2018; pmid: 29412908). Consequently, one of the potential areas where this antibody will be particularly useful is the food industry and food control against adulteration with meat products.
Antibody first published in:
Jiang et al. Monoclonal antibody-based ELISA for the quantification of porcine hemoglobin in meat products Food Chem. 2018 Jun 1;250:170-179. doi: 10.1016/j.foodchem.2018.01.032. Epub 2018 Jan 6. PMID:29412908
Note on publication:
Describes the generation and characterization of the antibody 13F7 as well as the construction of an assay detecting blood in food products.