United Kingdom (UK)
Recombinant monoclonal antibody to hINSR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma IR 18-34.
UniProt Accession Number of Target Protein: P06213
Alternative Name(s) of Target: CD220; human insulin receptor; insulin receptor; HHF5; IR; 18 34
Immunogen: This antibody was raised by immunising BALB/c mice with purified human placental insulin receptor.
Specificity: This antibody recognises the extracellular domain of human INSR. It does not cross-react with rat, mouse or rabbit insulin receptors, and displays very low cross-reactivity with bovine, porcine and ovine INSR.
Application Notes: This antibody has been used in immunoprecipitation assays, with both radiolabelled human insulin receptor (hINSR) and hINSR-radiolabelled insulin complexes (Soos et al, 1986). This antibody has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986) and immunohistochemistry. This antibody has been shown to have limited inhibitory effect on insulin binding to IM-9 cells (Soos et al, 1986). However, this antibody has been shown to stimulate insulin receptor autophosphorylation and kinase activity towards exogenous substrates (O'Brien et al, 1987).
Antibody first published in:
Soos et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1; 235(1): 199–208. PMID:2427071
Note on publication:
Describes the original generation of this antibody, and the characterisation of its specificity.