United Kingdom (UK)
Recombinant monoclonal antibody to HIV-1 GP120. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 0.5B.
Alternative Name(s) of Target: Gp120; Human immunodeficiency virus 1; Envelope glycoprotein gp120
Immunogen: The original antibody was prepared by immunization of BALB/c mice, first with heat inactivated whole viruses and second with a booster dose of purified antigen i.e.
Specificity: This antibody binds an epitope between amino acid residues 308 to 331 located in the V3 loop of the HIV-1 IIIB family of viruses.
Application Notes: 0.5 Beta is a neutralizing antibody that binds the external envelope glycoprotein gp120 of the HIV-1. The binding specificity of this antibody was checked using flow cytometry. The epitope to which 0.5 beta binds has homologous sequences with inter-alpha-trypsin inhibitor (Koito et al., 1989; PMID:2484964). This antibody in conjugation with immunotoxins like ricin A chain (RAC) or pseudomonas exotoxin (PE) was used to selectively inhibit proliferation and kill HIV infected cells. The humanized version of 0.5 beta retained both the binding affinity and neutralizing activity compared to its parent murine antibody and chimeric antibody. Furthermore this antibody was used for an ELISA on a synthetic 20 amino acid peptide (308-327) based on the sequence of the RP135 region of gp120 from HIV-IIIB (Maeda et al., 1991; PMID: 1873503). This antibody was used for a western blot on HTLV-IIIB antigens (Matsushita et al., 1988; PMID:2452899), and on Purified HTLV-IIIB (Skinner et al., 1988; PMID:2456088). The NMR structure of this antibody was determined (Tugarinov et al., 2000; PMID:10801487). The ability of this antibody to neutralize a cell-free HIV infection of H9 cells was assayed. It was indeed able to prevent this infection (Matsushita et al., 1988; PMID:2452899). This antibody was also able to neutralize the HIV infection of AA-5 cells (Skinner et al., 1988; PMID:2456088). This antibody was able to prevent HIV infection in chimpanzees in vivo (Emini et al., 1992; PMID:1741059).
Antibody first published in: Patent US5827723. PMID: