United Kingdom (UK)
Recombinant monoclonal antibody to HIV-1 GP120. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the human B cell clone b12 (MT12).
UniProt Accession Number of Target Protein: P05877; P03377
Alternative Name(s) of Target: Glycoprotein 120; SU; surface protein gp120; env polyprotein; envelope glycoprotein gp16
Immunogen: The original antibody was isolated from a combinatorial Fab phage display library generated from a long term asymptomatic male infected with clade B strain of HIV-1. Later on the Fab antibody was converted to an IgG1 by cloning the variable regions into a vector for mammalian expression.
Specificity: This antibody binds CD4 binding site of the HIV-1 GP120.
Application Notes: Binding affinity of the Fab antibody and the IgG1 version towards HIV-1 gp120 was determined using ELISA (US5804440). This antibody is a potent neutralizer of HIV strains IIIB and MN. The IgG1 version of b12 antibody showed 50% neutralization titers of less than 40ng/ml for both IIIB and MN strain in a p24 reporter assay. b12 can neutralize about 75% of clade B strains of HIV-1 (those most common in North America), but it neutralizes less than 50% of other strains of HIV-1 found worldwide (PMID: 7973652). This antibody has also shown promise in complete protection against HIV-1 in hu-PBL-SCID mouse model by passive immunization. The in vivo protective dose against viral challenge was found to be 4.5-7mg/kg (PMID: 7662189). This antibody is also reported to neutralize HXBc2 HIV-1 strain (PMID: 7518527).
Antibody first published in:
Burton et al. Efficient neutralization of primary isolates of HIV-1 by a recombinant human monoclonal antibody. Science. 1994 Nov 11;266(5187):1024-7. PMID:7973652
Note on publication:
Describes the generation and potent neutralization abilities of this antibody.