United Kingdom (UK)
Recombinant monoclonal antibody to HIV capsid protein p24. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 13B5.
Alternative Name(s) of Target: P24; CA
Immunogen: The original antibody was generated by immunizing mice with recombinant HIV-1 capsid protein p24.
Specificity: The antibody specifically recognizes an epitope on the C-terminal domain of HIV capsid protein p24. In particular, the Fab recognizes a region in the C-terminal domain of p24 in the loop between helices H10 and H11 (residues 205–213, centered around Pro207).
Application Notes: p24 is the major capsid protein of HIV and plays an active structural role both as part of the Gag polyprotein and in its processed form during virus assembly, maturation and disassembly. The association and dissociation rates and affinity constant for the formation of the 13B5 Fab complex with RH24 (the recombinant HIV-1 capsid protein) have been determined by surface plasmon resonance measurements (with affinity of 29nM). (PMID:11080628) PLA particles with surface adsorbed antigens p24 and gp120 were studied as new vaccine carriers. Competition ELISA was performed to investigate the influence of PLA adsorption on integrity and accessibility at the epitope level. Nanoparticle-adsorbed protein p24 was recognized by the antibody, indicating that the antigenicity and the structural integrity of PLA-adsorbed antigens was extremely well conserved. (PMID: 16919350)
Antibody first published in:
Colominas et al. Head-to-tail dimers and interdomain flexibility revealed by the crystal structure of HIV-1 capsid protein (p24) complexed with a monoclonal antibody Fab. EMBO J. 1999 Mar 1;18(5):1124-36. PMID:10064580
Note on publication:
The paper describes the crystal structure of an intact molecule of HIV-1 capsid protein (p24) in complex with the antibody Fab fragment.