United Kingdom (UK)
Recombinant monoclonal antibody to HIV capsid protein p24. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CB4-1.
Alternative Name(s) of Target: P24; CA
Immunogen: The original antibody was generated by immunizing Balb/c mice with purified recombinant p24 HIV-1 protein.
Specificity: The antibody binds the p24 capsid protein from HIV-1. The antibody recognizes specifically the linear epitope GATPQDLNTML comprising residues 46-56 of rp24.
Application Notes: The binding specificity of the monoclonal antibody CB4-1 is analyzed. Inhibition of CB4-1-p24 interaction by p24, UmuD, and candidapepsin were determined by competitive ELISA. Soluble proteins compete with solid phase-adsorbed p24 for CB4-1 binding. (PMID:9413989)
Antibody first published in:
Keitel et al. Crystallographic analysis of anti-p24 (HIV-1) monoclonal antibody cross-reactivity and polyspecificity. Cell. 1997 Dec 12;91(6):811-20. PMID:9413990
Note on publication:
The paper presents X-ray crystal structure of the Fab fragment of the antibody alone or in complex with four different peptides.