United Kingdom (UK)
Recombinant monoclonal antibody to HLA-DR. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma L243.
UniProt Accession Number of Target Protein: P04233
Alternative Name(s) of Target: CD74; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33
Immunogen: The original antibody was generated by immunizing mice with the human B cell line RPMI 8866.
Specificity: This antibody reacts with the HLA-DR antigen and does not cross react with HLA-DP or HLA-DQ. This antibody binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer. HLA-DR is an MHC class II cell surface receptor encoded by the human leukocyte antigen complex on chromosome 6 region 6p21.31. HLA-DR is also involved in several autoimmune conditions, disease susceptibility and disease resistance. The primary function of HLA-DR is to present peptide antigens, potentially foreign in origin, to the immune system for the purpose of eliciting or suppressing T-(helper)-cell responses that eventually lead to the production of antibodies against the same peptide antigen.
Application Notes: This antibody can be used for the immunoprecipitation of HLA-DR and MHC class-II peptides (PMID: 2961843; 11390619). L243 selectively inhibited the in vitro proliferation of Epstein-Barr virus-transformed lymphoblastoid cell lines (LCL) (PMID: 2467753). This antibody was used in a flow cytometric assay to detect the decrease in HLA-DR expression on monocytes which is an indicator of immunosuppression in patients with sepsis and is correlated with increased risk of secondary infection and mortality (PMID: 33432735). Alexa 568 and FITC labelled L243 antibody was used in the immunofluorescent staining of renal microvascular endothelial cells (RMEC) or human kidney tissue (PMID: 12707403). This antibody was also used in the detection of MHC class II protein in a western blot (PMID: 11390619). This antibody can also be used for the immunocytochemical and immunohistochemical analysis of frozen sections of necropsy brain tissue to identify HLA-DR expressing cells (PMID: 1864982).
Antibody first published in:
Lampson et al. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980 Jul;125(1):293-9. PMID:6966655
Note on publication:
Describes the generation of two antibodies against Ia-like molecules on the human B cell line Raji.