Anti-HPr [JEL42]

Description:

Recombinant monoclonal antibody to HPr. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma JEL42.

Clone Name:

JEL42

Published Application(s):

ELISA

Published Species Reactivity:

Escherichia coli

Products based on this clone:

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Ab03282-23.0 Anti-HPr [JEL42]
Escherichia coli
Rabbit IgG
Purified
In Stock

Ab03282-1.1 Anti-HPr [JEL42]
Escherichia coli
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: P0AA04

Alternative Name(s) of Target: Histidine-containing phosphocarrier protein; ptsH; hpr; Phosphocarrier protein HPr; Histidine-containing protein

Specificity: This antibody binds the histidine containing phosphocarrier protein of Escherichia coli. It is an 85 amino acid phosphocarrier protein which is component of phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.

Application Notes: This antibody can detect mutant histidine binding protein in crude extracts. It was also capable of inhibiting PTS sugar phosphorylation assays, but the antibody-HPr complex could still be phosphorylated by enzyme I (PMID: 2438269)

Antibody first published in: Waygood et al. Characterization of mutant histidine-containing proteins of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium. J Bacteriol. 1987 Jun; 169(6): 2810–2818. PMID:2438269
Note on publication: Describes the characterization of mutant HPr protein of E. coli using two different antibodies.

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