- Ab00314-23.0 Anti-HSPB1 [SAIC-23C-35]
- Rabbit IgG
- In Stock
- Ab00314-1.1 Anti-HSPB1 [SAIC-23C-35]
- Mouse IgG1
- In Stock
Recombinant monoclonal antibody to HSPB1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-23C-35.
UniProt Accession Number of Target Protein: P04792
Alternative Name(s) of Target: Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSP27; HSP28
Immunogen: Peptide "VSLDVNHFAPDELTVK" derived from Heat shock protein beta-1, involved in stress resistance and actin organisation, conjugated to KLH.
Specificity: Recognises human HSPB1.
Application Notes: Original data characterizing this antibody may be found here .
Antibody first published in:
Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.
Flow cytometry using the Anti-HSPB1 antibody SAIC-23C-35 (Ab00314). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-23C-35 (Ab00314-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-HSPB1 antibody SAIC-23C-35 (Ab00314). HeLa(A) (0.03µg/ml), MCF7(B) (0.001µg/ml), Caco-2(C) (0.1µg/ml) and A431(D) (0.03µg/ml) cell lysates (35µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-23C-35 (Ab00314-23.0), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of HeLa cells with anti-HSPB1 (Ab00314) SAIC-23C-35 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-23C-35 (Ab00314-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00314-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.