Anti-estrogen receptor alpha [D547]

Name: Anti-estrogen receptor alpha [D547]
SKU: Ab02566
Availability: InStock

Description:

Recombinant monoclonal antibody to estrogen receptor alpha. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma D547.

Clone Name:

D547

Published Application(s):

EIA, functional assays, IP, ELISA, IF, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab02566-7.1 Anti-estrogen receptor alpha [D547]
Human
Rat IgG2a
Purified
Ships in 5-6 weeks

Ab02566-7.4 Anti-estrogen receptor alpha [D547]
Human
Rat IgG2a
Fc Silent™
Purified
Ships in 5-6 weeks

Ab02566-10.0 Anti-estrogen receptor alpha [D547]
Human
Human IgG1
Purified
Ships in 5-6 weeks

Ab02566-10.3 Anti-estrogen receptor alpha [D547]
Human
Human IgG1
Fc Silent™
Purified
Ships in 5-6 weeks

Ab02566-23.0 Anti-estrogen receptor alpha [D547]
Human
Rabbit IgG
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P03372

Alternative Name(s) of Target: Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1
Immunogen: This antibody was raised by immunizing a Lewis rat, with purified estradiol-receptor complex of calf uterine nuclei

Specificity: This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling.

Application Notes: crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). An EIA was preformed on cytosol from breast cancer tumors using this antibody (Cren et al, 1991; pmid:1911441). COS-7 cells cotransfected with expression plasmids encoding ERa and HA-tagged ERb were immunoprecipitated with this antibody (Ogawa et al, 1998; pmid:9473491). Sucrose Density Gradient Analysis was preformed on GH3 cells (Tate et al, 1984; pmid:6708522). Immunocytochemistry was preformed on the reproductive tract of Female cynomolgus macaques using this antibody (McClellan et al, 1984; pmid:6373239). [3H]E2-labeled macaque E2 receptors were examined on sucrose density gradients using this antibody (McClellan et al, 1984; pmid:6373239).

Antibody first published in: Greene et al. Monoclonal antibodies to estrophilin: Probes for the study of estrogen receptors Proc Natl Acad Sci U S A. 1980 Jan;77(1):157-61. PMID:6928610
Note on publication: The paper describes the antibody being raised by immunizing lewis rats with purified estradiol-receptor complex of calf uterine nuclei.

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