United Kingdom (UK)
Recombinant monoclonal antibody to Human IgG. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 17F12.
Alternative Name(s) of Target: Immunoglobulin G
Immunogen: This antibody was raised against human IgG.
Specificity: This antibody binds to all four human IgG subtypes (IgG1, IgG2, IgG3 & IgG4). Some cross-reactivity with rabbit IgG, cynomolgus monkey IgG1 and rhesus macaque IgG1 has also been reported.
Application Notes: A Fab and an scFv fragment derived from this antibody, conjugated with bacterial alkaline phosphatase, have been used in sensitive enzyme immunoassays for the detection of human IgG (Carrier et al, 1995). Further, the antibody reacted with human IgG1, cynomolgus monkey IgG1 and rhesus macaque IgG1 by ELISA analysis, but it did not show any cross reactivity with cynomolgus monkey IgG4.
Antibody first published in:
Carrier et al. Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection Journal of Immunological Methods, 1995, Vol.181(2), pp.177-186 PMID:7745247
Note on publication:
Describes the derivation of an scFv and Fab from this antibody, and their use in a sandwich ELISA.
ELISA using 17F12 (Ab01176) and Rhesus macaque IgG1, Cynomolgus monkey IgG1, Cynomolgus monkey IgG4 and Human IgG1. Binding curves of the mouse IgG1 chimeric version of the Anti-Human IgG antibody 17F12 (Ab01176-1.1; orange line) and isotype control (Ab00102-1.1, anti-fluorescein; dotted orange line) to an ELISA plate coated with Rhesus macaque IgG1 (Ab00102-26.0, Absolute Antibody) at a concentration of 2.5 ug/ml, of the mouse IgG1 chimeric version of the Anti-Human IgG antibody 17F12 (Ab01176-1.1; blue line) and isotype control (Ab00102-1.1, anti-fluorescein; dotted blue line) to an ELISA plate coated with Cynomolgus monkey IgG1 (Ab00102-35.0, Absolute Antibody) at a concentration of 2.5 ug/ml, of the mouse IgG1 chimeric version of the Anti-Human IgG antibody 17F12 (Ab01176-1.1; purple line) and isotype control (Ab00102-1.1, anti-fluorescein; dotted purple line) to an ELISA plate coated with Cynomolgus monkey IgG4 (Ab00102-36.0, Absolute Antibody) at a concentration of 2.5 ug/ml and of the mouse IgG1 chimeric version of the Anti-Human IgG antibody 17F12 (Ab01176-1.1; green line) and isotype control (Ab00102-1.1, anti-fluorescein; dotted green line) to an ELISA plate coated with Human IgG1 (Ab00102-10.0, Absolute Antibody) at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Ab00854-1.1 and an anti-mouse IgG was used as secondary antibody for detection.