Anti-Human Muscle Acetylcholine Receptor [mAb 192]

Description:

Recombinant monoclonal antibody to Human Muscle Acetylcholine Receptor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mAb 192.

Clone Name:

mAb 192

Published Application(s):

Blocking, WB, ELISA

Published Species Reactivity:

Rat, Human, Mouse

8

Products based on this clone:

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Ab00431-1.1 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Mouse IgG1
Purified
In Stock

Ab00431-1.4 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Mouse IgG1
Fc Silent™
Purified
In Stock

Ab00431-1.7 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Mouse F(ab)2
AbFab2™ His-Tagged
Purified
Ships in 4-5 weeks

Ab00431-10.6 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Human Fab fragment
His-Tagged
Purified
Ships in 4-5 weeks

Ab00431-2.0 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Mouse IgG2a
Purified
In Stock

Ab00431-2.3 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Mouse IgG2a
Fc Silent™
Purified
In Stock

Ab00431-8.1 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Rat IgG2b
Purified
Ships in 4-5 weeks

Ab00431-8.4 Anti-Human Muscle Acetylcholine Receptor [mAb 192]
Rat, Human, Mouse
Rat IgG2b
Fc Silent™
Purified
In Stock

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UniProt Accession Number of Target Protein: P02708 (For the alpha-subunit)

Alternative Name(s) of Target: AChR
Immunogen: Human muscle AChR.

Specificity: The antibody binds to human muscle AChR with a Kd of 10 pM, to mouse muscle AChR with a Kd of 50 pM, and to rat muscle AChR with a Kd of 646 nM.

Application Notes: This antibody binds to the human muscle Acetylcholine receptor, which is a ligand-gated ion channel, transducing an action potential into the muscle cells. The antibody binds to the main immunogenic region (MIR) and prevents ligand-binding.

Antibody first published in: Kontou et al. Characterisation, crystallisation and preliminary X-ray diffraction analysis of a Fab fragment of a rat monoclonal antibody with very high affinity for the human muscle acetylcholine receptor. FEBS Letters 1996; 389(2):195-198 PMID:8766828
Note on publication: Describes the generation of a super-high-affinity rat antibody against human muscle AChR and subsequent crystallography.

Binding Curve

IF staining of HeLa cells using Ab00431-1.1. Click on image for more details.

Binding Curve

Immunofluorescence staining of fixed HeLa cells with anti-Human Muscle Acetylcholine Receptor antibody mAb 192 (Ab00431) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of mAb 192 (Ab00431-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00431-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.

Binding Curve

WB on mouse skeletal muscle tissue lysate using Ab00431-1.1. Click on image for more details.

Binding Curve

Western Blot using anti-AChR (Human Muscle Acetylcholine Receptor) antibody mAb 192 (Ab00431) Mouse skeletal muscle lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of mAb 192 (Ab00431-1.1) at 0.03 µg/ml before detection using an anti-mouse IgG1 secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for AChR is 58.9 kDa. Ab00431-1.1 successfully detected AChR in mouse skeletal muscle tissue lysate. Though this antibody is targetted to the human AChR it also reacts with mouse AChR.