United Kingdom (UK)
Recombinant monoclonal antibody to IDO1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 19C5.
UniProt Accession Number of Target Protein: P14902
Alternative Name(s) of Target: IDO-1; IDO; INDO; Indoleamine 2 3-dioxygenase 1; Indoleamine-pyrrole 2 3-dioxygenase; CGMCC NO 20770; EPR20374
Immunogen: The original antibody was generated by immunizing mice with recombinant human IDO1 protein.
Specificity: This antibody binds an enzyme called indoleamine 2,3-dioxygenase 1 (IDO1). This molecule catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. It is involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. It acts as a suppressor of anti-tumor immunity. IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral and bacterial infections.
Application Notes: This antibody can bind human IDO1 in an ELISA. The original mouse IgG1 antibody binds human IDO1 with an affinity constant of 7.68 X 10^(9) M^(-1). This antibody can be used for the immunohistochemical analysis of various normal and cancerous human tissues like lymph node cells and Hodgkin's lymphoma tissues. The immunohistochemical staining pattern of this antibody is similar to another anti-IDO1 antibody EPR20374 (CN113045667).
Antibody first published in: PMID:
Western blot using anti-IDO1 antibody 19C5 (Ab03196). Human lymph node (A) (0.1µg/ml), tonsil (B) (0.1µg/ml), placenta (C) (0.003µg/ml), and lungs (D) (0.03µg/ml) tissue lysates (35µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 19C5 (Ab03196-23.0) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.