United Kingdom (UK)
Recombinant monoclonal antibody to IL-1B. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybrodoma 1.
UniProt Accession Number of Target Protein: O73909
Alternative Name(s) of Target: Interleukin IL1‐beta; chIL-1B-1; Interleukin-1; chIL-1B
Immunogen: The original antibody was raised immunising BALB/C mice with recombinant chicken IL-1B protein.
Specificity: The antibody is specific for chicken IL-1β, a pleiotropic molecule with a wide spectrum of activities including metabolic, physiologic, hematologic, and immunologic effects. The antibody did not react with chicken IL-18.
Application Notes: The specificity of the original format of this antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 25037821). The antibody detected chIL-1β protein by Western blot analysis. The antibody was used as a capture antibody for measuring serum IL-1B levels in chicken by capture ELISA analysis. The original format of the antibody was used to localize IL-1β-secreting cells in chicken immune tissues. The antibody neutralized the bioactivity of chIL-1B-induced cell proliferation and NO production by primary thymocytes (Lee et al, 2014; pmid: 25037821).
Antibody first published in:
Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken IL-1β Poult Sci. 2014;93(9):2193‐2198. PMID:25037821
Note on publication:
The paper describes the generation and characterization of the antibody, which is specific for chIL-1β.
Western blot using anti-IL-1B antibody 1 (Ab02591). Chicken blood leucocytes either untreated (A) or LPS treated (B) lysates (35µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the mouse version of 1 (Ab02591-3.0) at 2µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence