Anti-IL-2 [LNKB-2]

Description:

Recombinant monoclonal antibody to IL-2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma LNKB-2.

Clone Name:

LNKB-2

Published Application(s):

IP, WB, ELISA, IF

Published Species Reactivity:

Human

Products based on this clone:

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Ab00232-1.1 Anti-IL-2 [LNKB-2]
Human
Mouse IgG1
Purified
In Stock

Ab00232-23.0 Anti-IL-2 [LNKB-2]
Human
Rabbit IgG
Purified
In Stock

Ab00232-13.12 Anti-IL-2 [LNKB-2]
Human
Human IgG4-S228P
Purified
In Stock

UniProt Accession Number of Target Protein: P60568

Alternative Name(s) of Target: Interleukin 2; LNKB2

Specificity: Recognises human Interleukin 2.

Antibody first published in: Fokin et al. Spatial structure of a Fab-fragment of a monoclonal antibody to human interleukin-2 in two crystalline forms at a resolution of 2.2 and 2.9 angstroms Bioorg Khim. 2000 Aug;26(8):571-8. PMID:11040993

WB on human PBM, spleen, thymus, Jurkat and Molt4 cell samples using Ab00232-23.0. Click on image for more details.

Western Blot using anti-IL-2 antibody LNKB-2 (Ab00232) Untreated human peripheral blood monocyte (PBM) (A), PMA/lonomycin-treated PBM (B), human spleen (C), human thymus (D), Jurkat cell (E) and Molt4 cell (F) lysate samples (35µg protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of LNKB-2 (Ab00232-23.0) at 1 µg/ml (A, B) or 0.1 µg/ml (C-F) before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected running size for IL-2 is 17.6kDa. Ab00232-23.0 successfully detected Il-2 in multiple human tissue / cell samples.