United Kingdom (UK)
Recombinant monoclonal antibody to IL-6. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybrodoma 1.26.4.
UniProt Accession Number of Target Protein: Q90YI0
Alternative Name(s) of Target: Interleukin 6; Interleukin-6; ChIL-6; chicken IL-6
Immunogen: The original antibody was raised by immunising BALB/C mice with recombinant chicken IL-6.
Specificity: This antibody binds specifically to chicken IL-6, an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine (cytokine produced from muscle). Further, the antibody shows cross-reactivity with human and murine IL-6.
Application Notes: The specificity of this antibody (IgG2b) towards the native protein chIL-6 was confirmed in ELISA analysis (Scott et al, 2006; pmid: 16930722). The antibody detected the denatured chIL-6 by western blot. The antibody showed inhibitory activity on murine IL-6 on a B9 hybridoma cell bioassay. The bioassay was run with varying concentrations of the antibody in order to determine the effective concentration for neutralizing IL-6. Further, recombinant forms of chicken, human and murine IL-6 were all tested in the bioassay. The antibody was effective in neutralizing all three recombinant IL-6 proteins. (Scott et al, 2006; pmid: 16930722).
Antibody first published in:
Scott et al. Monoclonal antibodies against chicken interleukin‐6. Vet Immunol Immunopathol. 2006;114(1‐2):173‐177 PMID:16930722
Note on publication:
The paper describes the generation and characterization of the antibody, which is specific for chIL-6.