Anti-IL-9R [AH9R2]

Description:

Recombinant monoclonal antibody to IL-9R. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AH9R2.

Clone Name:

AH9R2

Published Application(s):

Blocking, IP

Published Species Reactivity:

Human

Products based on this clone:

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Ab01263-3.0 Anti-IL-9R [AH9R2]
Human
Mouse IgG2b
Purified
In Stock

Ab01263-3.3 Anti-IL-9R [AH9R2]
Human
Mouse IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01263-23.0 Anti-IL-9R [AH9R2]
Human
Rabbit IgG
Purified
In Stock

Ab01263-10.0 Anti-IL-9R [AH9R2]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab01263-10.3 Anti-IL-9R [AH9R2]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab01263-2.0 Anti-IL-9R [AH9R2]
Human
Mouse IgG2a
Purified
In Stock

UniProt Accession Number of Target Protein: Q01113

Alternative Name(s) of Target: Interleukin-9 receptor; IL9R; CD129
Immunogen: This antibody was raised by immunising DBA/2 mice with P815 mastocytoma cells transfected with human IL-9R cDNA.

Specificity: This antibody binds specifically to the human IL-9 receptor alpha chain.

Application Notes: This antibody has been used to immunoprecipitate human IL-9R expressed by murine BW5147 cells transfected with hIL-9R-encoding cDNA (Demoulin et al, 1996). This antibody binds to and blocks the human IL-9Ra, and displays intermediate inhibitory activity; in a TS1H9RA3 proliferation assay, half-maximal inhibition of 100 pg/ml human IL-9 requires 10 mg/ml of this antibody (de Smedt et al, 2000). Equally, treatment with this antibody partially reduces the cellularity of human thymocytes in chimeric human-mouse FTOC, with no effect on murine thymocytes (de Smedt et al, 2000).

Antibody first published in: Demoulin et al. A single tyrosine of the interleukin-9 (IL-9) receptor is required for STAT activation, antiapoptotic activity, and growth regulation by IL-9. Mol Cell Biol. 1996 Sep;16(9):4710-6. PMID:8756628
Note on publication: Describes the original generation of this antibody, and its use in immunoprecipitation analysis.

If analysis of Jurkat cells using Ab01263-23.0. Click on image for more details.

Immunofluorescence staining of Jurkat cells using anti-IL-9R antibody (Ab01263) AH9R2. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of AH9R2 (Ab01263-23.0) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01263-23.0, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (Ab00102-23.0) followed by Alexa Fluor® 488 secondary antibody.

Flow cytometry on Jurkat cells using Ab01263-23.0. Click on image for more details.

Flow-cytometry using the Anti-IL-9R antibody AH9R2 (Ab01263). Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of HB-8592 (Ab01263-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.