Anti-IL2R [AHT54]

Name: Anti-IL2R [AHT54]
SKU: Ab00902
Availability: InStock

Description:

Recombinant monoclonal antibody to IL2R. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AHT54.

Clone Name:

AHT54

Published Application(s):

Block, ELISA, FC

Published Species Reactivity:

Human

Products based on this clone:

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Ab00902-1.1 Anti-IL2R [AHT54]
Human
Mouse IgG1
Purified
In Stock

Ab00902-23.0 Anti-IL2R [AHT54]
Human
Rabbit IgG
Purified
In Stock

Ab00902-13.12 Anti-IL2R [AHT54]
Human
Human IgG4-S228P
Purified
Ships in 4-5 weeks

Ab00902-10.0 Anti-IL2R [AHT54]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab00902-10.3 Anti-IL2R [AHT54]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

Ab00902-2.0 Anti-IL2R [AHT54]
Human
Mouse IgG2a
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P01589

Alternative Name(s) of Target: CD25; Interleukin 2 receptor; IL-2R; IL2 receptor; IL-2 receptor; Tac antigen; p55; AHT-54; AHT 54
Immunogen: BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.

Specificity: AHT54 recognises a determinant close to the IL-2 binding site.

Application Notes: AHT54 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988). AHT54 has also been shown to have anti-proliferative activities, and chimeric AHT54 antibodies with a human contant region have an increased inhibitory effect on proliferation of T lymphocytes (Rose 1992).

Antibody first published in: Osawa H et al. Studies on the formation of high-affinity IL-2 binding sites of an IL-2 receptor p55 + p75 heterodimeric complex: functional importance of a determinant on the p55 subunit defined by a monoclonal antibody AHT-107 Immunology Letters, 20 (1989) 205 - 212 Elsevie PMID:2469650
Note on publication: Describes the of binding monoclonal antibodies AHT107 and AHT54 to IL-2R and their inhibitory effect on IL-2 dependent proliferation of human T lymphoblasts.

Flow cytometry analysis of Jurkat cells using Ab00902-23.0. Click on image for more details.

Flow cytometry using the Anti-IL2R antibody AHT54 (Ab00902). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of AHT54 (Ab00902-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.