United Kingdom (UK)
Recombinant monoclonal antibody to inactivated vaccinia virus. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody VACC-E7.
Alternative Name(s) of Target: killed vaccinia virus; VV; VACV
Immunogen: Two llamas were immunized with a combination of killed vaccinia virus material and purified recombinant L1 antigen. A VHH library was then constructed and panned against killed vaccinia virus .
Specificity: The antibody is specific for vaccinia virus. The antibody does not recognize the L1 antigen. Vaccinia virus is a large, complex, enveloped virus belonging to the poxvirus family.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody was assayed as both capture and tracer in MAGPIX bead-based sandwich assays for the detection of vaccinia virus and a limit of detection (LOD) of 4x10^5 pfu/ml was achieved (Walper et al., 2014; PMID: 25211488).
Antibody first published in:
Walper et al. Development and evaluation of single domain antibodies for vaccinia and the L1 antigen PLoS One. 2014 Sep 11;9(9):e106263. PMID:25211488
Note on publication:
The original paper describes the generation and characterization of the antibody.