- Ab03960-23.0 Anti-Insulin receptor [BBBB138]
- Rabbit IgG
- Ships in 5-6 weeks
Recombinant monoclonal antibody to Insulin receptor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody BBBB138.
UniProt Accession Number of Target Protein: P06213
Alternative Name(s) of Target: IR
Immunogen: The original antibody was isolated from a human phage display library by panning against IR.
Specificity: The antibody is specific for the insulin receptor. The antibody recognises both short isoform (IR-A) and a long isoform (IR-B). The epitope of the antibody does not overlap with the insulin binding site.
Application Notes: The specificity of the antibody for IR was determined by ELISA analysis. The binding affinity of the antibody to IR-A and IR-B was measured by surface plasmon resonance (Kd= 2.57 and 2.98 nM respectively). The antibody blocked insulin induced IR phosphorylation in a dose-dependent manner on HuH7 cells. The antibody effectively decreased insulin induced glucose uptake at concentrations of 1 nM and 10 nM in vitro. The antibody induced hyperglycemia in C57 mice in in vivo experiments.
Antibody first published in: PMID: