United Kingdom (UK)
Recombinant monoclonal antibody to Integrin alpha 4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HP1/2.
UniProt Accession Number of Target Protein: P13612
Alternative Name(s) of Target: CD49d; VLA-4; α4; VLA4; ITGA4; α4β1; α4 integrin; VLA4 heterodimer (α4β1); Integrin alpha-4; Integrin alpha-IV; VLA-4 subunit alpha; CD49 antigen-like family member D; B1 integrin very late activation antigen-4
Specificity: This antibody specifically binds epitope B of the alpha 4 chain of human integrin alpha 4 (CD49d). Integrins alpha-4/beta-1 (VLA-4) acts as a receptor for fibronectin and VCAM1. VLA-4 is atypical because it is mostly restricted to lymphoid and myeloid cells, and indirect evidence had suggested that it might be involved in various cell-cell interactions. In addition, VLA-4 has been shown to mediate T and B lymphocyte attachment to the heparin II binding fragment of human plasma fibronectin (FN). This antibody is also reported to cross react with integrin alpha-4/beta-7 (α4β7).
Application Notes: This antibody can be used for the immunoprecipitation of VLA-4 from cell lysates (PMID: 2430809). This antibody is also capable of detecting integrin alpha 4 expressed on the surface of Ramos cells by flow cytometry (PMID: 2430809; 1689216). This antibody can also block adherence of CTT leukocytes to recombinant soluble VCAM 1-coated plates in vitro (PMID: 7686922). This antibody can also block the interaction of VLA-4 with fibronectin. This antibody does not cause cell aggregation (PMID: 1709929). This antibody can bind soluble integrin alpha-4 in an ELISA. This antibody also inhibited the binding of PAF-stimulated eosinophils to MAdCAM-1 transfectants. This antibody also inhibited binding of unstimulated eosinophils to soluble recombinant human (rh)VCAM-1 and fibronectin (Fn) (PMID: 8911148).
Antibody first published in:
Sánchez-Madrid et al. VLA-3: a novel polypeptide association within the VLA molecular complex: cell distribution and biochemical characterization. Eur J Immunol. 1986 Nov;16(11):1343-9. PMID:2430809
Note on publication:
Describes the generation of eight antibodies against VLA-4 antigen.
Immunofluorescence staining of Jurkat cells with anti-Integrin alpha 4 antibody HP1/2 (Ab03253). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of HP1/2 (Ab03253-23.0) (1:100 dilution) for 1 hour followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left to right, top to bottom, Ab03253-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.