Anti-Integrin alpha 5 beta 1 [SG/19]

Description:

Recombinant monoclonal antibody to Integrin alpha 5 beta 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SG/19.

Clone Name:

SG/19

Published Application(s):

functional assay, IP, FC, IF

Published Species Reactivity:

Human

Products based on this clone:

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Ab03564-23.0 Anti-Integrin alpha 5 beta 1 [SG/19]
Human
Rabbit IgG
Purified
Ships in 5-6 weeks

Ab03564-1.1 Anti-Integrin alpha 5 beta 1 [SG/19]
Human
Mouse IgG1
Purified
Ships in 5-6 weeks

Ab03564-10.0 Anti-Integrin alpha 5 beta 1 [SG/19]
Human
Human IgG1
Purified
Ships in 5-6 weeks

Ab03564-10.3 Anti-Integrin alpha 5 beta 1 [SG/19]
Human
Human IgG1
Fc Silent™
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P05556

Alternative Name(s) of Target: CD29; ITGB1; FNRB; MDF2; MSK12; ITGA5:ITGB1; Integrin alpha V beta1; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta
Immunogen: The original antibody was generated by immunizing BALB/c mice with Nalm-6 cells.

Specificity: This antibody binds human integrin alpha 5 beta 1 near the N-terminus. Epitope mapping of SG/19 revealed that Thr82 in the 1 subunit, located at the outer face of the boundary between the I-like and hybrid domains, was the key binding determinant for this antibody. Integrin alpha5 beta 1 is also known as fibronectin receptor and binds matrix macromolecules and proteinases and thereby stimulates angiogenesis. Integrin α5β1, is a member of the integrin family, is a heterodimer composed by two subunits, α5 (ITGA5/CD49e) and β1 (ITGB1/CD29), and both are necessary for complete biological functions. The interaction of VLA-5 with fibronectin plays an important role in regulating inflammatory cytokine production by human articular chondrocytes (From the Cell Migration Gateway ITGA5 ITGB1

Application Notes: This antibody was capable of recognizing integrin alpha 5 beta 1 expressed on the surface of K562 cells via immunofluorescence staining. This antibody also precipitated molecules with weight of 110 and 140 kDa under nonreducing conditions from K562 cells and Ramos cells, and 130 kDa when reduced. These results were similar to the ones reported for integrin alpha 5 beta 1. The effect of this antibody on migration beneath stromal cells was investigated by inoculating Nalm-6 and BMS2 cells together with this antibody. It was seen that the presence of this antibody blocked the migration of Nalm-6 cells beneath stromal cells (PMID: 1400595).

Antibody first published in: Miyake et al. Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture. J Cell Biol. 1992 Nov;119(3):653-62. PMID:1400595
Note on publication: This paper discloses a study on physical interactions between human lymphomas and murine bone marrow derived stromal cells using antibodies.