Anti-Integrin alpha-L [D2-57]

Description:

Recombinant monoclonal antibody to Integrin alpha-L. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display library D2-57.

Clone Name:

D2-57

Published Application(s):

crystallography, in vitro, SPR, ELISA, FC, IF

Published Species Reactivity:

Human

UniProt Accession Number of Target Protein: P20701

Alternative Name(s) of Target: AL-57; CD11a; ITGAL; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain
Immunogen: The original antibody was raised using human phage display library FAB-300. To screen the library purified locked-open HA, locked-closed LA, and wild-type I domains of LFA-1 were used.

Specificity: This antibody is specific for the LFA-1 I domain in a HA conformation.

Application Notes: The original antibody was used for an ELISA on LFA-1 HA I domain. This antibody was used for immunofluorescence on K562 cells with different LFA-1 conformations. Furthermore this antibody was used for flow cytometry on K562 cells with different LFA-1 conformations (Huang et al., 2006; PMID:16888085), and on IL-15-cultured T lymphocytes (Shimaoka et al., 2006; PMID:16963559). This antibody was used for an ICAM-1 blocking assay on HA cells, to determine the effect of the antibody to inhibit ligand binding. The antibody was used for a PHA-induced lymphocyte proliferation assay on PBMCs, to determine the effect of the antibody on these cells. It did indeed block PHA-induced lymphocyte proliferation (Huang et al., 2006; PMID:16888085). This antibody was used for surface plasmon resonance on LFA-1 I domain to determine its binding affinity (Shimaoka et al., 2006; PMID:16963559). The structure of this antibody was determined using x-ray crystallography (Zhang et al., 2009; PMID:19805116).

Antibody first published in: Huang et al. Identification and characterization of a human monoclonal antagonistic antibody AL-57 that preferentially binds the high-affinity form of lymphocyte function-associated antigen-1 J Leukoc Biol. 2006 Oct;80(4):905-14. PMID:16888085
Note on publication: The original paper reports the identification and characterization of a human antibody AL-57, which binds to the alpha(L) I domain in a HA but not LA conformation.

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