Anti-Interferon gamma [XMG1.2]

Description:

Recombinant monoclonal antibody to Interferon gamma. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma XMG1.2.

Clone Name:

XMG1.2

Published Application(s):

Blocking, in vivo, neutralization, ELISA, FC

Published Species Reactivity:

Mouse

UniProt Accession Number of Target Protein: P01580

Alternative Name(s) of Target: IFN gamma; IFN-gamma; interferon-γ; IFN-γ; IFNG; IFG; IFI; IMD69
Immunogen: The original antibody was generated by immunizing a Lewis rat with soluble recombinant mouse IFN-γ.

Specificity: This antibody is specific for mouse interferon gamma.

Application Notes: The original antibody (rat IgG1, κ) was effective in both ELISA and two blocking bioassays (Cherwinski et al., 1987; PMID: 2960769). This antibody was used successfully in flow cytometry (Sander et al., 1993; PMID: 8288874). This antibody was used in vivo to block and neutralize IFN-γ (Suzuki and Joh, 1994; PMID: 8202451) (Beatty and Paterson, 2001; PMID: 11160282).

Antibody first published in: Cherwinski et al. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987 Nov 1;166(5):1229-44. doi: 10.1084/jem.166.5.1229 PMID:2960769
Note on publication: The original publication explores the lymphokine synthesis patterns of T cell clones, distinguishing between two types (Th1 and Th2) based on mRNA hybridization, revealing differences in the production of specific cytokines and providing insights into the functional specificity of these clones using monoclonal antibodies and bioassays.

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