United Kingdom (UK)
Recombinant monoclonal antibody to JIP-2/IB-2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma N135/37.2.
UniProt Accession Number of Target Protein: Q9ERE9
Alternative Name(s) of Target: N135/37.2R; N135/37; C-Jun-amino-terminal kinase-interacting protein 2; JIP-2;
JNK-interacting protein 2; Islet-brain-2;
IB-2; JNK MAP kinase scaffold protein 2; Mitogen-activated protein kinase 8-interacting protein 2
Immunogen: This antibody was raised by immunising BALB/c mice with Fusion protein corresponding to Mouse IB-2 aa 226-421.
Specificity: This antibody is specific for a sequence of amino acids between the 226th and 421th amino acid of the JIP-2 gene. The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAP
Application Notes: This antibody is recommended for the detection and analysis of JIP-2/IB-2 by western blot and immunohistochemistry. For instance, the mouse version of this antibody was used to detect JIP-2/IB-2 by western blot in adult rat brain membranes. And it was also used to detect JIP-2/IB-2 by immunohistochemistry in adult rat brain membranes.
Antibody first published in:
Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360
Note on publication:
This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research.