United Kingdom (UK)
Recombinant monoclonal antibody to KRAS G12C mutant. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma AbAbKRAS01.
UniProt Accession Number of Target Protein: P01116
Alternative Name(s) of Target: K-Ras; GTPase Kras; KRAS2; RASK2; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; ras p21 protein; p21 ras
Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human KRAS G12C mutant protein.
Specificity: This antibody binds the G12C mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer.
Application Notes: The binding reactivity of the original antibody (mouse IgG1) antibody towards mutated KRAS (G12C), VEGFA, TIGIT, HB-EGF and HSP90 was tested using ELISA. It was reported that this antibody does not cross-react with any other molecule than mutant KRAS. This antibody specifically recognized the KRAS protein in celly lysates of human lung cancer cell line A549 and human colon cancer cell line SW480 in a western blot (CN111705065).
Antibody first published in: PMID: