United Kingdom (UK)
Recombinant monoclonal antibody to LAM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BJRbL03.
Alternative Name(s) of Target: Lipoarabinomannan; Mycobacterium cell wall polysaccharide
Immunogen: This antibody was generated by immunizing a rabbit with Mycobacterium tuberculosis H37Rv cell wall.
Specificity: This antibody is specific for the LAM protein of Mycobacterium tuberculosis but shows cross-reactivity with other Mycobacterium species. M. tuberculosis causes the disease tuberculosis.
Application Notes: The ability of the rabbit version of this antibody to bind LAM was determined using ELISA on LAM. Furthermore, the affinity of this antibody to LAM was determined using a ForteBio Octe machine. Finally, the ability of this antibody to distinguish isolates of M. tuberculosis (MTB), M. avium-intracellulare (MAI), M. abscessus, and M. fortuitum-abscessus (MA-MF) was assessed using flow cytometry; it bound all bacteria (Yan et al., 2021; PMID: 32146163). This antibody was used as a capture antibody in a sandwich ELISA (Yan et al., 2023; PMID: 37764103).
Antibody first published in:
Yan et al. Generation of mycobacterial lipoarabinomannan-specific monoclonal antibodies and their ability to identify mycobacterium isolates. J Microbiol Immunol Infect. 2021 Jun;54(3):437-446. PMID:32146163
Note on publication:
Novel rabbit monoclonal antibodies (mAbs) against LAM for immunodetection purposes were developed.