United Kingdom (UK)
Recombinant monoclonal antibody to LAM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CS-35.
Alternative Name(s) of Target: Lipoarabinomannan
Immunogen: The original antibody was generated against LAM from Mycobacterium leprae.
Specificity: This antibody binds lipoarabinomannan and recognizes the common terminal branched saraf-containing arrangement.
Application Notes: This antibody has been used as a reference antibody for the characterization of mAbs against LAM and to study a variety of cellular functions, such as the role of LAM in the interaction of mycobacteria with macrophages. This antibody can recognize LAM from Mycobacterium tuberculosis strain Erdman and H37Ra and Mycobacterium bovis in a western blot assay (Chatterjee et al., 1992; PMID: 1556132) (Prinzis et al., 1993; PMID: 8277248). This antibody was also used for the study of the interaction of LAMs with lymphocytes and monocytes using immunofluorescence assays and flow cytometry (Ilangumaran et al., 1995; PMID: 7636199). Epitope mapping of this antibody was done using ELISA (Kaur et al., 2002; PMID: 12368438). The binding of this antibody to LAM has been thoroughly characterized (Rademacher et al., 2007; PMID: 17672460).
Antibody first published in:
Chatterjee et al. Lipoarabinomannan of Mycobacterium tuberculosis. Capping with mannosyl residues in some strains. J Biol Chem. (1992); 267(9):6234-9. PMID:1556132
Note on publication:
Describes the use of this antibody to show the difference in antigenicity of basic LAM and mannose-capped LAM.