United Kingdom (UK)
Recombinant monoclonal antibody to LDL receptor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 12G4.
UniProt Accession Number of Target Protein: P01130
Alternative Name(s) of Target: LDLR; Low density lipoprotein receptor
Immunogen: The original antibody was generated by immunizing mice with a peptide corresponding to amino acid residues 195-222 (CDGGPDCKDKSDEENCAVATCRPDEFQC) of the human LDL receptor.
Specificity: This antibody recognizes and binds an epitope between amino acid residues 195-222 of the human low density lipoprotein receptor.
Application Notes: The binding specificity of this antibody was detected using ELISA. This antibody is reported to be used to identify LDL-R expression level in cancer cell lines like HepG2, HeLa, MCF-7, Jurkat, Ramos, HuH7, A549, MDA-MB-231 and Hek293 using flow cytometry. It was found that HepG2 and HeLa cells express LDL-R most strongly. This antibody is also reported to cross react with mouse, rat and hamster LDL receptors. It strongly cross reacts with C2C12, CHO-K1, and YB2/0 cells. 12G4 antibody binding site is the same as that of LDLs and it competes with LDLs for binding site on A549 cells. This antibody was also used for the immunohistochemical analysis of LDL-receptors on cells from the colon, testes, pancreas, gastric mucosa, the basal cell layer of the buccal and exocervical mucosa, and the various renal tubules. It was also used for immunohistochemical analysis of mammary adenocarcinomas (US20090175789).
Antibody first published in: US20090175789 PMID: