Anti-LMP2A [14B7]

Name: Anti-LMP2A [14B7]
SKU: Ab01294
Availability: InStock

Description:

Recombinant monoclonal antibody to LMP2A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 14B7.

Clone Name:

14B7

Published Application(s):

IB, IP, WB, ELISA, IF

Published Species Reactivity:

Epstein-Barr virus

Products based on this clone:

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Ab01294-7.1 Anti-LMP2A [14B7]
Epstein-Barr virus
Rat IgG2a
Purified
In Stock

Ab01294-23.0 Anti-LMP2A [14B7]
Epstein-Barr virus
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: A8CDV5

Alternative Name(s) of Target: Latent membrane protein 2A
Immunogen: This antibody was raised by immunising Lou/C rats with a bacterial trpE-LMP2A fusion protein.

Specificity: This antibody is specific for Epstein-Barr virus LMP2A, and reacts with an epitope encompassing amino acids 36 to 64.

Application Notes: The specificity of this antibody for LMP2A has been confirmed in ELISA analysis and immunofluorescence and immunoblot analysis with a baculovirus-expressed LMP2A protein (Fruehling et al, 1996). This antibody has also been used in immunoprecipitation, immunofluorescence and immunoblot analysis of LMP2A-transfected BJAB cell lysates (Fruehling et al, 1996; Rovedo et al, 2007) and lymphoblastoid cell lines transformed with LMP2AY112F mutant EBV (Fruehling et al, 1998). Additionally, this antibody has been used in Western blot analysis of whole cell lysates of LMP2A-transfected BJAB, HaCat and HEK293 cells (Anderson et al, 2008).

Antibody first published in: Fruehling et al. Identification of latent membrane protein 2A (LMP2A) domains essential for the LMP2A dominant-negative effect on B-lymphocyte surface immunoglobulin signal transduction. J Virol. 1996 Sep;70(9):6216-26. PMID:8709248
Note on publication: Describes the original generation of this antibody, and the characterisation of its specificity in immunoprecipitation, immunofluorescence and immunoblot analysis.

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