United Kingdom (UK)
Recombinant monoclonal antibody to LPS. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody M18F.
Alternative Name(s) of Target: Lipopolysaccharide; Burkholderia spp; Burkholderia mallei; BM; glanders
Immunogen: The original antibody was generated by immunizing mice with various antigen preparations of B. mallei (American Type Culture Collection [ATCC] 23344). Later on phage display technique was used to generate scFv version of this antibody.
Specificity: This antibody binds surface lipopolysaccharides of Burkholderia mallei. B. mallei (BM), a host-adapted pathogen that does not normally persist in nature, causes glanders in horse. Some studies indicated that BM is highly infectious in humans by aerosol route.
Application Notes: This antibody can be used for the detection of Burkholderia mallei lipopolysaccharide in a sample using ELISA (PMID: 21172353)
Antibody first published in:
Kim et al. Construction and molecular characterization of mouse single-chain variable fragment antibodies against Burkholderia mallei and Burkholderia pseudomallei. J Immunol Methods. 2011 Feb 28;365(1-2):101-9. PMID:21172353
Note on publication:
Describes the molecular charcterization of scFv antibodies against Burkholderia mallei and Burkholderia pseudomallei generated using scFv phage display technology.