United Kingdom (UK)
Recombinant monoclonal antibody to M protein, serotype 5. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 24.1.2.
UniProt Accession Number of Target Protein: P02977
Alternative Name(s) of Target: Myosin; Streptococcal M-protein; Actin; Tropomyosin; Troponin
Immunogen: The original antibody was generated by immunizing BALB/c mice with streptococcal M type 5 membranes followed by a second, boosting dose of Lysin M5.
Specificity: This antibody reacts with α-helical molecules and is anti-nuclear (binds DNA). Specifically, it binds streptococcal M-protein (type 5), human myosin, actin, tropomyosin, and troponin.
Application Notes: This antibody binds myosin at a low relative avidity, as determined in a competitive ELISA (Mertens et al., 2000; PMID: 11282394).
Antibody first published in:
Cunningham et al. Human and murine antibodies cross-reactive with streptococcal M protein and myosin recognize the sequence GLN-LYS-SER-LYS-GLN in M protein J Immunol. 1989 Oct 15;143(8):2677-83 PMID:2677144
Note on publication:
The original publication describes the use of overlapping synthetic peptides to map the myosin cross-reactive epitopes of streptococcal M protein recognized by antibodies from acute rheumatic fever and rheumatic heart disease sera.