United Kingdom (UK)
Recombinant monoclonal antibody to M protein, serotype 5. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 27.4.1.
UniProt Accession Number of Target Protein: P02977
Alternative Name(s) of Target: Myosin; Streptococcal M-protein; Keratin; Tropomyosin; Ganglioside; Glycophorin M,N
Immunogen: The original antibody was generated by immunizing BALB/c mice with streptococcal M type 5 membranes followed by a second, boosting dose of Lysin M5.
Specificity: This antibody reacts with α-helical molecules. Specifically, it binds streptococcal M-protein (type 5), human myosin, keratin, tropomyosin, ganglioside, and glycophorin M,N.
Application Notes: This antibody reacts with the human retina, as shown in an indirect immunofluorescence assay. This antibody reacts with retinal S-antigen (S-Ag), as shown in an ELISA (Lerner et al., 1995; PMID: 8722579). This antibody binds myosin at a high relative avidity, as determined in a competitive ELISA. Reactivity with glycophorin M and N was determined by hemagglutination assay (Mertens et al., 2000; PMID: 11282394).
Antibody first published in:
Cunningham et al. Human and murine antibodies cross-reactive with streptococcal M protein and myosin recognize the sequence GLN-LYS-SER-LYS-GLN in M protein J Immunol. 1989 Oct 15;143(8):2677-83 PMID:2677144
Note on publication:
The original publication describes the use of overlapping synthetic peptides to map the myosin cross-reactive epitopes of streptococcal M protein recognized by antibodies from acute rheumatic fever and rheumatic heart disease sera.