United Kingdom (UK)
Recombinant monoclonal antibody to Major capsid protein L1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma H16.1A.
UniProt Accession Number of Target Protein: P03101
Alternative Name(s) of Target: L1; Human papillomavirus type 16
Immunogen: The original antibody was raised by immunizing a BALB/c mouse with HPV-16 or HPV-18 L1 VLPs.
Specificity: This antibody is specific for a conformational epitope covering amino acids 266–297 and 339–365.
Application Notes: The fab version of this antibody was used a neutralization assay, in short both a pre- and post-virus attachment assay was performed on 293TT cells with pseudo virus. The structure of this antibody was determined using cryo-EM (Guan et al., 2015; PMID:25996608). This antibody was used for surface plasmon resonance on virus like particles (Towne et al., 2013; PMID:23159495). This antibody was used for an ELISA on HPV-16 L1wt VLPs. This antibody was used for a neutralization assay on HPV-16 pseudo virions, in short the antibody was incubated with HPV-16 pseudo virions, then an attempt was made to infect cervical carcinoma C33A cells with the mixture. This antibody showed some neutralization (Christensen et al., 2001; PMID:11878901).
Antibody first published in:
Christensen et al. Surface Conformational and Linear Epitopes on HPV-16 and HPV-18 L1 Virus-like Particles as Defined by Monoclonal Antibodies Virology. 1996 Sep 1;223(1):174-84. PMID:8806551
Note on publication:
The original paper generated a panel of 24 monoclonal antibodies (MAbs) against human papillomavirus (HPV) types 16 and 18 L1 virus-like particles (VLPs).