United Kingdom (UK)
Recombinant monoclonal antibody to Major capsid protein L1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma H16.U4.
UniProt Accession Number of Target Protein: P03101
Alternative Name(s) of Target: L1; Human papillomavirus type 16
Immunogen: The original antibody was raised by immunizing a BALB/c mouse with HPV-16 or HPV-18 L1 VLPs.
Specificity: This antibody is specific for amino acids 427 to 445 of the C-terminal arm of L1.
Application Notes: The original antibody was used for an ELISA on intact papillomavirus and virus like particles contained L1 protein of different papillomavirus genotypes, and on L1 protein (Guan et al., 2015; PMID:26401038), on HPV quasivirus (Guan et al., 2017; PMID:29211035), and on HPV virus like particles (Carter et al., 2003; PMID:14557648). Further this antibody was used for a western blot on L1 protein (Christensen et al., 1996; PMID:8806551). The structure of this antibody was determined using cryo-EM (Guan et al., 2015; PMID:26401038, Guan et al., 2017; PMID:29211035).
Antibody first published in:
Christensen et al. Surface Conformational and Linear Epitopes on HPV-16 and HPV-18 L1 Virus-like Particles as Defined by Monoclonal Antibodies Virology. 1996 Sep 1;223(1):174-84. PMID:8806551
Note on publication:
The original paper generated a panel of 24 monoclonal antibodies (MAbs) against human papillomavirus (HPV) types 16 and 18 L1 virus-like particles (VLPs).