United Kingdom (UK)
Recombinant monoclonal antibody to MBP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the synthetic construct sAb-P1.
UniProt Accession Number of Target Protein: P0AEY0; P0AEX9
Alternative Name(s) of Target: malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein
Immunogen: This conformationally selective synthetic antibody was generated by customized phage display selections that stabilize MBP in different conformational states.
Specificity: This antibody is a conformation specific antibody and binds the closed conformation of MBP across the face of the closed binding pocket, effectively trapping the maltose inside. It does not bind the open conformation of the MBP. This antibody binds the peristeric site of MBP with its epitope spanning across the two lobes that form the entrance to the maltose-binding pocket. A short α-helix comprising residues 64–73 on one lobe of MBP and the long helix formed by residues 334–352 on its other lobe, form part of the extended interface between sAB-P1 and MBP. These two helices have a remarkably different relative orientation in the open and closed forms of MBP, thereby imparting conformational specificity of sAB-P1.
Application Notes: The affinity of maltose towards MBP is enhanced more than 1000-fold in the presence of an excess of sAB-P1. However, it is reported to inhibit the maltose transport by the Escherichia coli maltose transporter, MalFGK2, across the periplasm. This is because the epitope for sAB-P1 is different from the other sABs (sAB-7O and sAB-11M). It is also confirmed that sAB-P1 provides additional stabilization of the maltose-bound state (PMID: 29321208).
Antibody first published in:
Mukherjee et al. Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins. J Biol Chem. (2018); 293(8); 2815-2828. PMID:29321208
Note on publication:
Describes the conformational structure and characterization of this antibody.