United Kingdom (UK)
Recombinant monoclonal antibody to mCherry. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes LAM-4.
UniProt Accession Number of Target Protein: Q5S3G8; X5DSL3
Alternative Name(s) of Target: mCh; RFP; Fluorescent protein raspberry; Red fluorescent proteins; MCherry fluorescent protein; DsRed; RFP-RANbody-4; LaM4; RFP nanobody; RNb
Immunogen: The original antibody was generated by immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA library was generated by high-throughput DNA sequencing of a marrow lymphocyte from an immunized llama. Later on this was combined with mass spectrometric (MS) identification of high affinity VHH regions derived from serum of the same animal.
Specificity: This antibody binds mCherry and can also bind DsRed.
Application Notes: This antibody can be used to detect mCherry fluorescent protein and DsRed in an ELISA. This antibody binds immobilized mCherry with a binding affinity of Kd= 0.18 nM. The Alexa Fluor® 488 conjugated version of this antibody was used to immunofluorescently stain S. cerevisiae expressing mCherry-tagged histone H2B (PMID: 25362362). This antibody was used for the visualization and manipulation of red fluorescent labelled proteins in live cells by using nanobodies targeting fluorescent protein tags (PMID: 31122229).
Antibody first published in:
Fridy et al. A robust pipeline for rapid production of versatile nanobody repertoires. Nat Methods. 2014 Dec; 11(12): 1253–1260. PMID:25362362
Note on publication:
Describes the generation of large repertoires of readily expressible recombinant nanobodies with high affinities and specificities against a given antigen.