United Kingdom (UK)
Recombinant monoclonal antibody to mCherry. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes LAM-6.
UniProt Accession Number of Target Protein: Q5S3G8; X5DSL3
Alternative Name(s) of Target: mCh; RFP; Fluorescent protein raspberry; Red fluorescent protein; MCherry fluorescent protein; DsRed; tdTomato; LAM6
Immunogen: The original antibody was generated by immunization of Llama glama with mCherry fluorescent protein. A VHH cDNA library was generated by high-throughput DNA sequencing of a marrow lymphocyte from an immunized llama. Later on this was combined with mass spectrometric (MS) identification of high affinity VHH regions derived from serum of the same animal.
Specificity: This antibody binds mCherry and can also bind DsRed and tdTomato, which is a tandem dimer of mCherry.
Application Notes: This antibody reacts with mCherry and also recognizes dsRed2, and tdTomato. This antibody binds immobilized mCherry with a binding affinity of Kd= 0.26 nM (PMID: 25362362).
Antibody first published in:
Fridy et al. A robust pipeline for rapid production of versatile nanobody repertoires. Nat Methods. 2014 Dec; 11(12): 1253–1260. PMID:25362362
Note on publication:
Describes the generation of large repertoires of readily expressible recombinant nanobodies with high affinities and specificities against a given antigen.