United Kingdom (UK)
Recombinant monoclonal antibody to mouse Bim. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma ham151-149.
UniProt Accession Number of Target Protein: O54918
Alternative Name(s) of Target: Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death
Immunogen: The original antibody was generated from a hamster immunized with a complex of mouse Bim and Bcl-XL produced in insect cells.
Specificity: The antibody reacts with mouse Bim. Mouse Bim is a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses.
Application Notes: The antibody reacted in Western blots and by immunofluorescence with permeabilized B and T cells from Bim +/+ mice (Oliver et al., 2004; PMID: 15520248). T cells were analyzed for levels of Bcl-2 and Bim protein by staining of permeabilized cells with the antibody (Jorgensen et al., 2007; PMID: 17785775). The antibody was used for flow cytometry analysis (Kashiwakura et al., 2013; PMID: 23278598).
Antibody first published in:
Oliver et al. Loss of Bim Allows Precursor B Cell Survival But Not Precursor B Cell Differentiation in the Absence of Interleukin 7. J Exp Med. 2004 Nov 1;200(9):1179-87. PMID:15520248
Note on publication:
The original paper describes the generation and characterization of the antibody.